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| Coelho,Stefânia Vilas Boas; Rosa,Sttela Dellyzete Veiga Franco da; Fantazzini,Tatiana Botelho; Baute,Júlia Lima; Silva,Luciano Coutinho. |
| ABSTRACT Coffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drying in silica gel; Recalcitrant seeds; Ice crystals; Isoenzymes; Physiological quality. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000600588 |
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| Santos, Paulo Augusto Almeida; Paiva, Renato; Silva, Luciano Coutinho; Souza, Ana Cristina de; Santana, Marlucia Cruz de; Silva, Diogo Pedrosa Corrêa da. |
| The aim of this study was to evaluate the efficiency of vitrification and droplet vitrification for the cryopreservation of Hancornia speciosa shoot tips. The shoot tips were subjected to four different periods of exposure (15, 30, 45 and 60 min.) to plant vitrification solution 2 (PVS2) before plunging into liquid nitrogen. We evaluated the regrowth of H. speciosa shoot tips that were cryopreserved by the classical vitrification technique and by the droplet vitrification technique. Shoot tips were submitted to different periods of pre-culture (absence, 24 or 48h) in a medium containing 0.3 M sucrose prior to cryopreservation. With a PVS2 exposure period of 60 min., significant differences were observed between the two techniques used in this study with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.00.00.00-4 - ciências agrárias; 2.03.03.00-9 - fisiologia vegetal; Native species; In vitro conservation; Recalcitrant seeds; Plant genetic diversity conservation; Vitrification; Droplet vitrification Conservação in vitro; Criopreservação vegetal. |
| Ano: 2015 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19573 |
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| Faria,José M.R.; Davide,Lisete C.; Silva,Edvaldo A. A. da; Davide,Antonio C.; Pereira,Roselaine C.; van Lammeren,André A.M.; Hilhorst,Henk W.M.. |
| The remarkably short storability of recalcitrant seeds has prevented their inclusion in programs of ex situ conservation. The causes of their desiccation sensitivity and rapid decline in viability during storage are not fully elucidated yet. In this study the highly recalcitrant, fully viable embryos of the tree species Inga vera subsp. affinis were stored under various conditions and analyzed physiologically and cytologically at intervals, in order to obtain more insights in their loss of viability during storage. Embryos stored fully hydrated at 5ºC fully lost viability in 18 d. Sealed storage of partially dehydrated embryos slightly prolonged their viability, with 95% germination being attained after 14 d of storage. However, after 30 d of storage,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abscisic acid; Polyethylene glycol; Recalcitrant seeds; Seed conservation. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000400008 |
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| MARTINS,CIBELE CHALITA; NAKAGAWA,JOÃO; BOVI,MARILENE LEÃO ALVES. |
| Com o objetivo de identificar a tolerância à dessecação de sementes de Euterpe espiritosantensis, dois lotes de frutos foram colhidos na coleção de palmeiras do Instituto Agronômico de Campinas, no município de Ubatuba, estado de São Paulo e transportados em embalagem impermeável até a Faculdade de Ciências Agronômicas/UNESP, município de Botucatu, estado de São Paulo, onde foram despolpados. As sementes foram submetidas à secagem em sílica gel a partir das testemunhas não desidratadas (51,4% e 46,6% de teor de água), retirando-se amostras a cada 12 ou 24 horas. O efeito da desidratação foi avaliado através da porcentagem de germinação, e da velocidade de protrusão de embrião, do botão germinativo, da plúmula, e do comprimento das plântulas. Avaliaram-se... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Palmae; Recalcitrant seeds; Drying. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84041999000300007 |
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